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Isolation PCR-ready DNA from samples containing PCR inhibitors

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00020501" target="_blank" >RIV/00216224:14310/07:00020501 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Isolation PCR-ready DNA from samples containing PCR inhibitors

  • Original language description

    Magnetic nonporous hydrophilic P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres containing carboxyl groups were used for purification of DNA isolated from the lyophilisates of four strains of Trichophyton fungi (without cultivation). The purified DNA wassuitable for amplification in PCR-based methods. The composition of lysis buffers and a method of spore destruction were also tested.

  • Czech name

    Izolace DNA v kvalitě pro PCR ze vzorků obsahujících inhibitory PCR

  • Czech description

    Magnetické neporézní hydrofilní mikročástice P(HEMA-co-EDMA) a P(HEMA-co-GMA), obsahující karboxylové skupiny, byly použity pro purifikaci DNA izolované z lyofilizátů čtyř kmenů houby Trichophyton (bez kultivace).Purifikovaná DNA byla v kvalitě vhodné pro PCR. Bylo rovněž optimalizováno složení lyzačních pufrů a testovány metody rozrušení spór.

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2007

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Biopowders; Advances in the Powder Technology

  • ISBN

    978-963-9696-23-5

  • ISSN

  • e-ISSN

  • Number of pages

    6

  • Pages from-to

    159-164

  • Publisher name

    Research Institute of Chemical and Process Enginnering, University of Pannonia, Vesazprém, Hungary

  • Place of publication

    Budapest, Hungary

  • Event location

    Budapest

  • Event date

    Sep 13, 2007

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article