Isolation, Cultivation and Identification of Borrelia burgdorferi genospecies from Ixodes ricinus ticks from South Moravia, Czech Republic.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00021763" target="_blank" >RIV/00216224:14310/07:00021763 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Isolation, Cultivation and Identification of Borrelia burgdorferi genospecies from Ixodes ricinus ticks from South Moravia, Czech Republic.

Original language description

The prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from South Moravia was investigated. A total of 305 ticks (21 larvae, 243 nymphs, 19 females and 22 males) were collected in suburban woods of Pisárky Park (city of Brno) from Julyto October 2002. All specimens were examined by dark-field microscopy (DFM) for the presence of spirochetes. The mean infection rate was 14.8%. 13.6% of males and 26.3% of females tested were positive for the presence of B. burgdorferi s.l.. Also, of the 243 nymphs, 14.4% were positive and out of 21 larvae 9.5% were positive. Borreliae were identified by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) methods. From a total of 45 DFM positive samples 37 were positive for the presence of borrelian DNA. Isolation attempts resulted in 21 spirochaetal strains. Identification by PCR-RFLP detection revealed genospecies: Borrelia afzelii, B. garinii, B. burgdorferi sensu stricto and B. valaisiana. Four isolated s

Czech name

Izolace, kultivace a identifikace genospecies Borrelia burgdorferi z klíšťat Ixodes ricinus na jižní Moravě, Česká republika.

Czech description

Na jižní Moravě byla zjišťována promořenost klíšťat druhu Ixodes ricinus bakterií Borrelia burgdorferi sensu lato. Od července do října 2002 bylo v lesích na parkové lokalitě Pisárky v Brně shromážděno 305 klíšťat (21 larev, 243 nymf, 19 samic a 22 samců). Všechny vzorky byly vyšetřeny zástinovou mikroskopií (DFM) na přítomnost spirochet. Průměrná pozitivita byla 14,8%. Bylo pozitivních 13,6% samců a 26,3% samic, 14,4% a 9,5% nymf. Borrelie byly identifikovány metodami PCR-RFLP. Ze 45 DFM pozitivních vzorků bylo 37 pozitivních na přítomnost borreliové DNA. Podařilo se získat 21 izolátů. Výsledkem analýzy PCR-RFLP bylo určení těchto genospecies: Borrelia afzelii, B. garinii, B. burgdorferi sensu stricto a B. valaisiana. 4 izolované kmeny ukázalay směsnou infekci těchto genospecies.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Annals of Agricultural and Environmental Medicine

ISSN

1232-1966

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

1

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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