A new tool for distinguishing of different structural forms of lactoferrin

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00022110" target="_blank" >RIV/00216224:14310/07:00022110 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
A new tool for distinguishing of different structural forms of lactoferrin
Original language description
Lactoferrin is considered a multifunctional or multi-tasking protein. It appears to play several biological roles, whereas its structure is very crucial. The aim of this work was to investigate basic electrochemical behaviour of lactoferrin by both stationary and flow electrochemical methods with respect to study of structural changes of the compound of interest. The behaviour of lactoferrin was studied by three various electrochemical methods (linear sweep, differential pulse and square wave voltammetry). Based on the results obtained, we utilized flow injection analysis with electrochemical detection for determination of lactoferrin. We found out that the most suitable FIA-ED conditions were as follows: working electrode potential of 900 mV, Britton-Robinson buffer (pH 4.5) as mobile phase, its flow rate 1 ml.min-1. The detection limit was about tens of ng per ml. Finally, we attempted to follow the changes of lactoferrin signal in the presence of chemical compounds or under the phys
Czech name
Nový nástroj pro rozdělení rozdílných strukturních forem laktoferinu
Czech description
Nový nástroj pro rozdělení rozdílných strukturních forem laktoferinu

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
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Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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