Analysis of hormone-dependent de novo organogenesis in Arabidopsis thaliana.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00041968" target="_blank" >RIV/00216224:14310/07:00041968 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Analysis of hormone-dependent de novo organogenesis in Arabidopsis thaliana.
Original language description
Mutual interactions of cytokinins and auxins were identified as a major morphogenetic factor in plants. Molecular mechanisms are still largely unknown. Hypocotyls derived from ethiolated seedlings were affected by various concentration ratios of exogenous auxins and cytokinins. We analysed a role of both phytohormones in an ability of hypocotyl explants to form calli, SAM- and/or RAM-like structures. We applied two auxins, NAA and 2,4-D, differing in the mechanism of their intercellular transport, and auxin transport inhibitor NPA. WUS::NLS-GUS and CLV3::NLS-GUS marker lines were used to recognize the identity of newly formed organs and the role of both phytohormones in the regulation of the cytokinin-to-auxin ratio-dependent developmental switch. Changes in endogenous hormone maxima and position-specific hormone-dependent mitotic cell activity were studied. Suggested model of the cytokinin and auxin interactions during de novo organogenesis in Arabidopsis thaliana will be presented.
Czech name
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Czech description
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Project
<a href="/en/project/LC06034" target="_blank" >LC06034: Regulation of morphogenesis of plant cells and organs</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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