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Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans

Result description

Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)5 primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)5-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)5 primer to be a fast and reliable method for identification of S. mutans.

Keywords

Dental caries(GTG)5-PCRIdentificationAutomated ribotypingStreptococcus mutans

The result's identifiers

Alternative languages

  • Result language

    angličtina

  • Original language name

    Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans

  • Original language description

    Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)5 primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)5-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)5 primer to be a fast and reliable method for identification of S. mutans.

  • Czech name

    (GTG)5-PCR pro rychlou identifikaci druhu Streptococcus mutans

  • Czech description

    Metoda rep-PCR s primerem (GTG)5 byla použita pro analýzu bakterií izolovaných ze zubního kazu dětí. Tyto kmeny byly dále analyzovány automatickou ribotypizací (RiboPrinter) a analýzou celkových buněčných proteinů. Na základě získaných výsledků se (GTG)5-PCR ukázala jako vhodná pro identifikaci druhu Streptococcus mutans.

Classification

  • Type

    Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    1M0528: Dental research centre

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology

  • ISSN

    0003-6072

  • e-ISSN

  • Volume of the periodical

    94

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    7

  • Pages from-to

  • UT code for WoS article

    000259670400008

  • EID of the result in the Scopus database

Basic information

Result type

Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

Jx

CEP

EE - Microbiology, virology

Year of implementation

2008