Inactivation of colicin Y by intramembrane helixhelix interaction with its immunity protein

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00027657" target="_blank" >RIV/00216224:14310/08:00027657 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/08:00320823

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Inactivation of colicin Y by intramembrane helixhelix interaction with its immunity protein

Original language description

Construction of hybrids between colicins U and Y and mutagenesis of colicin Y gene (cya) revealed amino acid residues important for interactions between colicin Y and its cognate immunity protein (Cyi). Four such residues (I578, T582, Y586, and V590) were found in helices 8 and 9 of the colicin Y pore forming domain. To verify the importance of these residues, the corresponding amino acids in the colicin B protein were mutated to the residues present in colicin Y. An E. coli strain with cloned colicin Yimmunity gene (cyi) inactivated this mutant but not the wildtype colicin B. In addition, interacting amino acid pairs in Cya and Cyi were identified using a set of Cyi point mutant strains. These data are consistent with antiparallel helix-helix interactions between Cyi helix T3 and Cya helix 8 of the pore-forming domain as a molecular mechanism of colicin Y inactivation by its immunity protein.

Czech name

Inaktivace kolicinu Y jeho imunitním proteinem

Czech description

Je popsána inaktivace kolicinu Y jeho imunitním proteinem, predevším pak jde o identifikaci aminokyselinových zbyků, které se podílí na interakci mezi kolicinem a jeho imunitním proteinem.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEBS Journal

ISSN

1742-464X

e-ISSN

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Volume of the periodical

275

Issue of the periodical within the volume

21

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

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UT code for WoS article

000260010600008

EID of the result in the Scopus database

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