New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00029161" target="_blank" >RIV/00216224:14310/09:00029161 - isvavai.cz</a>

Alternative codes found

RIV/25328859:_____/09:#0000423

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus

Original language description

In PI466495, a powdery mildew resistance source of wild barley (Hordeum vulgare ssp. spontaneum), one gene conferring powdery mildew resistance was identified in the Mla locus. In this paper, the RGH1a gene sequence was used as source for the developmentof a cleaved amplified polymorphic sequence (CAPS) marker. Cosegregation between this marker and powdery mildew resistance was analysed by specific DNA fragments associated with each allele of the gene using 286 F2 plants derived from a cross between winter barley (H. vulgare L.) variety Tiffany and PI466495. For the codominant marker RGH1aI1a, three fragments, 370 bp, 82 bp and 59 bp in size, were amplified from F2 plants exhibiting resistance reaction types 0 and 0/1 to powdery mildew; whereas two fragments, 429 bp and 82 bp in size, were amplified in susceptible plants. Simple procedures based on polymerase chain reaction and restriction enzyme digestion allowed for identifying the plants susceptible to powdery mildew (Blumeria gram

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA522%2F06%2F0608" target="_blank" >GA522/06/0608: Development of DNA markers and construction of a genetic map to localize new genes for powdery mildew resistance in winter barley</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cereal Research Communications

ISSN

0133-3720

e-ISSN

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Volume of the periodical

37

Issue of the periodical within the volume

1

Country of publishing house

HU - HUNGARY

Number of pages

7

Pages from-to

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UT code for WoS article

000264755800011

EID of the result in the Scopus database

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