Quantitative proteomic analysis of lung carcinoma cell line transfected by mutant p53 gene sequences
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00029724" target="_blank" >RIV/00216224:14310/09:00029724 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Quantitative proteomic analysis of lung carcinoma cell line transfected by mutant p53 gene sequences
Original language description
The present study constitutes the use of a quantitative LC-MS/MS based proteomic approach for the comparative proteomic analysis of lung carcinoma cell line H1299 steady transfected by two different mutant p53 gene sequences (hot-spot mutations at codonpositions 175 and 273, respectively). The proteomics analysis method involved iTRAQ 8-plex stable isotope labeling of tryptic peptides, their separation via off-line coupled two-dimensional liquid chromatography (SCX and RP-LC) followed by tandem mass spectrometry on the MALDI-TOF/TOF platform (iTRAQ-2DLC-MS/MS). The principal aims of this study were: (1) to define the protein spectrum detectable using this approach, and (2) to quantitative compare the proteomic profiles of cells, which differ only in type of p53 mutation after 17-(allylamino)-17-demethoxygeldanamycin (17-AAG) treatment. The study resulted in the reproducible identification of 411 non-redundant proteins.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů