Purine derivates - separation and characterization by selected methods

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00036147" target="_blank" >RIV/00216224:14310/09:00036147 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Purine derivates - separation and characterization by selected methods
Original language description
Purine bases represent the basic constituents of DNA, their analysis is useful in the diagnosis of several diseases and metabolite disorders. The related bases, such as 6-aminopurine (6-AP, 2-aminopurine (2-AP) and 2,6-diaminopurine (2,6-DAP) were studies by spectroscopic and RP-HPLC techniques. Studied APs can be separated based on their spectral properties. Elution order related to polarity of molecule, in MF MetOH/30mM phosphate buffer (15:85), was following: 2,6-DAP, 2-AP, 6-AP. The phosphate concentration is not crucial. Experimental pKa values of model APs were calculated from corrected capacity factor dependence of APs on pH. The basicity increasing in order 2-AP<6-AP<2,6-DAP. Studies of basic structures will be used for nucleosides and nucleotides study, where 2-AP and 2,6-DAP can be used as fluorescent labels.
Czech name
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Czech description
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Project
<a href="/en/project/LC06035" target="_blank" >LC06035: Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
IX. Pracovní setkání fyzikálních chemiků a elektrochemiků
ISBN
978-80-7375-309-2
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
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Publisher name
MZLU, Brno
Place of publication
Brno
Event location
MZLU, Brno
Event date
Jun 29, 2009
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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