Reciprocal and multi-species chromosome BAC painting in crucifers (Brassicaceae)
Result description
The paper reports on principles of multi-species and multi-directional painting in crucifer species (Brassicaceae). We have successfully tested chromosome-specific BAC contigs of A. thaliana (n = 5) and A. halleri (n = 8) as probes for reciprocal BAC painting. Furthermore,BAC contigs of both Arabidopsis species were applied as multi-species painting probes to a third crucifer species, Noccaea caerulescens (n = 7), revealing their shared chromosome homeology. Specifically, we found homeology across portions of chromosomes At2, Ah4, and Nc4, which reflects their shared common origin with chromosome AK4 of the Ancestral Crucifer Karyotype (n = 8). We argue that multispecies and multi-directional painting will significantly expedite comparative cytogenomics in Brassicaceae and other plant families.
Keywords
Arabidopsis halleriArabidopsis thalianaBAC FISHBrassicaceaeCollinearityComparative chromosome paintingPlant cytogenetics
The result's identifiers
Result code in IS VaVaI
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Reciprocal and multi-species chromosome BAC painting in crucifers (Brassicaceae)
Original language description
The paper reports on principles of multi-species and multi-directional painting in crucifer species (Brassicaceae). We have successfully tested chromosome-specific BAC contigs of A. thaliana (n = 5) and A. halleri (n = 8) as probes for reciprocal BAC painting. Furthermore,BAC contigs of both Arabidopsis species were applied as multi-species painting probes to a third crucifer species, Noccaea caerulescens (n = 7), revealing their shared chromosome homeology. Specifically, we found homeology across portions of chromosomes At2, Ah4, and Nc4, which reflects their shared common origin with chromosome AK4 of the Ancestral Crucifer Karyotype (n = 8). We argue that multispecies and multi-directional painting will significantly expedite comparative cytogenomics in Brassicaceae and other plant families.
Czech name
—
Czech description
—
Classification
Type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cytogenetic and Genome Research
ISSN
1424-8581
e-ISSN
—
Volume of the periodical
129
Issue of the periodical within the volume
1-3
Country of publishing house
CH - SWITZERLAND
Number of pages
6
Pages from-to
—
UT code for WoS article
000280519500020
EID of the result in the Scopus database
—
Basic information
Result type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP
EB - Genetics and molecular biology
Year of implementation
2010