Testing of the influenza virus purification by capillary isoelectric focusing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00042757" target="_blank" >RIV/00216224:14310/10:00042757 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Testing of the influenza virus purification by capillary isoelectric focusing

Original language description

In the virological practice the preconcentration, purification and subsequently the determination of the purity and the concentration of the viruses from the cultural medium and/or from the real sample are required. The conventional techniques used todayare equipment demanding, time-consuming and labourious. In this study user friendly virus purification from the biological sample and the determination of the resulting viral sample purity by the capillary isoelectric focusing with UV detection are introduced. The Equine and Swine influenza viruses from the infected allantoic fluid from specific pathogen free embryonated chicken eggs were precipitaded by PEG 6000 and sodium chloride. The precipitated viruses were sedimented already at the accelerationabout 14 000 g. The impurities with different densities were removed on the sucrose gradients. The efficiency of the virus purification technique was examined by real-time PCR and compared with the results from the capillary isoelectric f

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

31/2010

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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