Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00043430" target="_blank" >RIV/00216224:14310/10:00043430 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis
Original language description
Labeling of nucleic acids offers broader option for its biochemical and physicochemical studies by the analytical separation methods such as capillary electrophoresis. Suitable labeling allows monitoring of DNA hybridization as well as following of DNA interactions with other biological and/or non-biological compounds. Chemical DNA probe consisting of osmium tetroxide and 2,2'-bipyridine (Os,bipy) is well-known especially for its electrochemical activity. In contrary to vanishing reactivity with purines, Os,bipy reagent creates covalent adducts with pyrimidine bases. The fastest adduct formation was observed namely with thymine. In our work, we analyzed selected pentanucleotides with various number and position of thymine bases and we monitored its reactivity with Os,bipy reagent. Our interest was also taken to ageing of Os,bipy reagent and the role of reagent abundance in mixture with oligonucleotide was studied.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LC06035" target="_blank" >LC06035: Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Acta Universitatis Palackianae Olomucensis Chemica 47S
ISBN
978-80-244-2470-5
ISSN
0232-0061
e-ISSN
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Number of pages
158
Pages from-to
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Publisher name
Palacký University, Olomouc
Place of publication
Olomouc
Event location
Olomouc, Czech Republic
Event date
Feb 8, 2010
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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