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Electrochemical Detection of mRNA Isolated from Plant Tissues Using Paramagnetic Microparticles

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00044270" target="_blank" >RIV/00216224:14310/10:00044270 - isvavai.cz</a>

  • Alternative codes found

    RIV/62156489:43210/10:00160477

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    čeština

  • Original language name

    ELEKTROCHEMICKÁ DETEKCE mRNA IZOLOVANÉ Z ROSTLINNÝCH PLETIV ZA VYUŽITÍ PARAMAGNETICKÝCH MIKROČÁSTIC

  • Original language description

    The aim of this study was to utilize paramagnetic microparticles in isolation of mRNA using electrochemical detection. In this way mRNA from maize seedlings treated with a Cd(II) salt was isolated. The interaction of paramagnetic particles with syntheticoligo and polynucleotides of various chain lengths was investigated. The average detection limits were 0.020 to 0.360 nM and the quantification limits 0.080 to 1.200 nM.

  • Czech name

    ELEKTROCHEMICKÁ DETEKCE mRNA IZOLOVANÉ Z ROSTLINNÝCH PLETIV ZA VYUŽITÍ PARAMAGNETICKÝCH MIKROČÁSTIC

  • Czech description

    The aim of this study was to utilize paramagnetic microparticles in isolation of mRNA using electrochemical detection. In this way mRNA from maize seedlings treated with a Cd(II) salt was isolated. The interaction of paramagnetic particles with syntheticoligo and polynucleotides of various chain lengths was investigated. The average detection limits were 0.020 to 0.360 nM and the quantification limits 0.080 to 1.200 nM.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CG - Electrochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA102%2F08%2F1546" target="_blank" >GA102/08/1546: Miniaturized intelligent systems and nanostructured elektrodes for chemical, biological, and pharmaceutical applications (NANIMEL)</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2010

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Chemické Listy

  • ISSN

    0009-2770

  • e-ISSN

  • Volume of the periodical

    104/2010

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    9

  • Pages from-to

  • UT code for WoS article

  • EID of the result in the Scopus database