Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00045193" target="_blank" >RIV/00216224:14310/10:00045193 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS
Original language description
The human gastrointestinal tract contains a diverse microbial community. Among them lactobacilli are important members of the natural intestinal microbiota playing a beneficial role in balancing of the intestinal environment. A collection of 51 Lactobacillus isolates originating from children intestinal mucous tissues has been studied. Analysed biopsy samples were retrieved during a routine endoscopic examination of different parts of the large intestine in patients revealing various disorders. A combined approach, which included automated ribotyping and MALDI-TOF MS analysis, was applied for identification of isolated Lactobacillus spp. in order to compare the discriminatory power of these methods. Automated ribotyping using the RiboPrinter MicrobialCharacterization System (DuPont Qualicon) assigned 38 lactobacilli to the species level: Lactobacillus salivarius (29 isolates), Lactobacillus paracasei subsp. paracasei (7) and Lactobacillus rhamnosus (2).
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů