Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00049467" target="_blank" >RIV/00216224:14310/11:00049467 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity
Original language description
The lysosomal aspartic protease cathepsin D (cath-D) is often over-expressed in breast cancer and acts as a mitogen on both cancer and stromal cells. The ability of cath-D to stimulate cancer cell migration remains controversial. To determine whether cath-D expression modulates breast cancer cell migration in vitro, we performed a real-time analysis of MDA-MB-231 breast cancer cell migration using novel methodology based on impedance measurement that enables continuous monitoring of the transition of the cells through a microporous membrane [xCELLigence equipped with CIM-plate 16 (Roche)]. We observed that altering cath-D level by either siRNA or cDNA tranfections significantly affected migration of MDA-MB-231 cells. Tumor cells have often impaired classical caspase-dependent apoptosis pathway and may be therefore more sensitive to agents that trigger alternative cell death pathways. Targeting lysosomes represents a method of choice as many human tumors have increased levels of lysosom
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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