(GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00052382" target="_blank" >RIV/00216224:14310/11:00052382 - isvavai.cz</a>
Result on the web
<a href="http://www.springerlink.com/content/4241mw07j07k367w/" target="_blank" >http://www.springerlink.com/content/4241mw07j07k367w/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12223-011-0006-4" target="_blank" >10.1007/s12223-011-0006-4</a>

Result language
angličtina
Original language name
(GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women
Original language description
A group of lactobacilli isolated from the cervix of 31 healthy women was characterized by (GTG)5-polymerase chain reaction (PCR) fingerprinting in order to evaluate this method for identification of vaginal lactobacilli. Obtained fingerprints were compared with profiles available in an in-house database of the CCM bacteria collection covering type and reference strains of multiple lactic acid bacteria including lactobacilli. Selected strains representing individual clusters were further identified by pheS gene sequencing. In total, six lactobacillus species were found among lactobacilli isolated from the cervix of healthy women. The (GTG)5-PCR method identified Lactobacillus gasseri (11 strains), Lactobacillus fermentum (one), and some of the Lactobacillus jensenii strains (eight out of 11), but failed to identify the remaining strains, including the Lactobacillus crispatus (18), Lactobacillus mucosae (one), and Lactobacillus vaginalis (one) species. L.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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