Efficiency of PCR-based methods in discriminating Bifidobacterium longum ssp. longum and Bifidobacterium longum ssp. infantis strains of human origin

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00053293" target="_blank" >RIV/00216224:14310/11:00053293 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/11:00370661
Result on the web
<a href="http://dx.doi.org/10.1016/j.mimet.2011.06.014" target="_blank" >http://dx.doi.org/10.1016/j.mimet.2011.06.014</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mimet.2011.06.014" target="_blank" >10.1016/j.mimet.2011.06.014</a>

Result language
angličtina
Original language name
Efficiency of PCR-based methods in discriminating Bifidobacterium longum ssp. longum and Bifidobacterium longum ssp. infantis strains of human origin
Original language description
Four molecular-biological methods (PCRs, random amplified polymorphic DNA (RAPD) method using 5 primers, repetitive sequence-based (rep)-PCR with BOXA1R and (GTG)5 primers and amplified ribosomal DNA restriction analysis (ARDRA)) and biochemical analysiswere compared for the classification of B. longum strains isolated from the faeces of breast-fed healthy infants (25), healthy adults (3) and 2 collection strains. These methods together with arabionose/ melezitose fermentation analysis clearly differentiated only the collection strains into B. longum ssp. longum and B. longum ssp. infantis at the subspecies level. On the other hand, ARDRA analysis differentiated the strains into the B. longum/infantis subspecies using the cleavage analysis of genus-specific amplicon with just one enzyme, Sau3AI. Moreover, we propose a classification of the collection strains into the subspecies level: B. longum CCM 3764 as B. longum ssp. longum CCM 3764, B. longum ATCC 17930 as B. longum ssp. infantis
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/2B06053" target="_blank" >2B06053: New methods for the characterization and identification of probiotic bacterial strains suitable for functional foods.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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