Bisprimer?A Program for the Design of Primers for Bisulfite-Based Genomic Sequencing of Both Plant and Mammalian DNA Samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00059285" target="_blank" >RIV/00216224:14310/12:00059285 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/12:00383563

Result on the web

<a href="http://jhered.oxfordjournals.org/content/early/2012/01/13/jhered.esr137.abstract" target="_blank" >http://jhered.oxfordjournals.org/content/early/2012/01/13/jhered.esr137.abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jhered/esr137" target="_blank" >10.1093/jhered/esr137</a>

Result language

angličtina

Original language name

Bisprimer?A Program for the Design of Primers for Bisulfite-Based Genomic Sequencing of Both Plant and Mammalian DNA Samples

Original language description

Plants and animals differ in the sequence context of the methylated sites in DNA. Plants exhibit cytosine methylation in CG, CHG, and CHH sites, whereas CG methylation is the only form present in mammals (with an exception of the early embryonic development). This fact must be taken into account in the design of primers for bisulfite-based genomic sequencing because CHG and CHH sites can remain unmodified. Surprisingly, no user-friendly primer design program is publicly available that could be used to design primers in plants and to simultaneously check the properties of primers such as the potential for primer-dimer formation. For studies concentrating on particular DNA loci, the correct design of primers is crucial. The program, called BisPrimer, includes 2 different subprograms for the primer design, the first one for mammals and the second one for angiosperm plants. Each subprogram is divided into 2 variants.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Heredity

ISSN

0022-1503

e-ISSN

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Volume of the periodical

103

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

5

Pages from-to

308-312

UT code for WoS article

000300723400017

EID of the result in the Scopus database

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