Engineered recombinant enzyme haloalkane dehalogenase DhaA83
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00064440" target="_blank" >RIV/00216224:14310/12:00064440 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Engineered recombinant enzyme haloalkane dehalogenase DhaA83
Original language description
Thermostable haloalkane dehalogenase DhaA83 prepared by saturated mutagenesis. Thermostable haloalkane dehalogenase DhaA63 prepared by site-directed mutagenesis. Thermostable haloalkane dehalogenase DhaA61 prepared by site-directed mutagenesis. Mutationstargeting as few as four residues lining the access tunnel extended enzyme?s half-life in 40% dimethyl sulfoxide from minutes to weeks (4,000-fold) and increased its melting temperature by 19 C. Protein crystallography and molecular dynamics revealed that the tunnel residue packing is a key determinant of protein stability and the active-site accessibility for co-solvent molecules (red dots). The broad applicability of this concept was verified by analyzing twenty six proteins with buried active sitesfrom all six enzyme classes.The results was made based on the verification of properties.
Czech name
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Czech description
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Classification
Type
G<sub>funk</sub> - Functional sample
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED0001%2F01%2F01" target="_blank" >ED0001/01/01: CETOCOEN</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Internal product ID
LL_05_2012
Numerical identification
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Technical parameters
výrobní postup, funkční vzorek byl vyroben na základě ověření vlastností, licenční smlouva nebyla zatím uzavřena.
Economical parameters
MU, IČO00216224
Application category by cost
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Owner IČO
00216224
Owner name
MU
Owner country
CZ - CZECH REPUBLIC
Usage type
P - Využití výsledku jiným subjektem je v některých případech možné bez nabytí licence
Licence fee requirement
Z - Poskytovatel licence na výsledek nepožaduje v některých případech licenční poplatek
Web page
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