Caspase 3 chemiluminescence activity determination in apoptotic cells and design of caspase-3 sensor

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00072525" target="_blank" >RIV/00216224:14310/12:00072525 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Caspase 3 chemiluminescence activity determination in apoptotic cells and design of caspase-3 sensor
Original language description
Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development of tumor or autoimmune disorders. In some instances knowledges of this pathway can be used for inhibit or prevent cancer development andother diseases. One of the earliest and most consistent observed features of apoptosis is the induction of a series of cytosolic proteases - caspases. Active caspases cleave numerous intracellular proteins and contribute to apoptotic cell death. Caspasesrecognize tetra-peptide sequences Asp-Glu-Val-Asp on their substrates and hydrolyze peptide bonds after aspartic acid residues. It is known from literature that in one apoptotic cell about 1.6x10(-19) mol of caspase can be activated. Various techniquesfor the determination of caspase 3 in free cells or tissue samples are commercially available. Caspase 3 activity is usually assayed by a chemiluminescence reaction. The best commercial methods reach the limit o detection higher than 1 pg
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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