Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00065568" target="_blank" >RIV/00216224:14310/13:00065568 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1758-2229.2012.00378.x/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/j.1758-2229.2012.00378.x/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1758-2229.2012.00378.x" target="_blank" >10.1111/j.1758-2229.2012.00378.x</a>

Result language

angličtina

Original language name

Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies

Original language description

Staphylococcus aureus is a serious human and veterinary pathogen in which new strains with increasing virulence and antimicrobial resistance occur due to acquiring new genes by horizontal transfer. It is generally accepted that temperate bacteriophages play a major role in gene transfer. In this study, we proved the presence of various bacterial genes of the S. aureus COL strain directly within the phage particles via qPCR and quantified their packaging frequency. Nonparametric statistical analysis showed that transducing bacteriophages 11, 80 and 80lpha of serogroup B, in contrast to serogroup A bacteriophage 81, efficiently package selected chromosomal genes localized in 4 various loci of the chromosome and 8 genes carried on variable elements like staphylococcal cassette chromosome SCCmec, staphylococcal pathogenicity island SaPI1, genomic islands Saalpha and Sabeta, and plasmids with various frequency. Bacterial gene copy number per ng of DNA isolated from phage particles ranged be

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Environmental Microbiology Reports

ISSN

1758-2229

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

66-73

UT code for WoS article

000314474500006

EID of the result in the Scopus database

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