Molecular characterization of brewing and wine yeast strains in the Czech Republic

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00069170" target="_blank" >RIV/00216224:14310/13:00069170 - isvavai.cz</a>
Alternative codes found
RIV/60193697:_____/13:#0000894
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/yea.v30.S1/issuetoc" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/yea.v30.S1/issuetoc</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Molecular characterization of brewing and wine yeast strains in the Czech Republic
Original language description
The hybrid origin of lager yeasts causes problems in determination of the technologically diverse species S. pastorianus and S. cerevisiae. Wine and brewing yeast S. cerevisiae could be differentiated on the DNA level. In our study we chose FLO and HIS4gene, the regions ITS, RRL2 and YBR033w and mtDNA as target sequences. A group of 28 brewing, wine and type yeast strains was analyzed by PCR and RFLP techniques. Detection of flocculation genes did not document any species-specific representation in anyof the strain under study. Gene LgFLO, considered as typical for lager yeast, was detected also in ale yeast. Other analyses led to an identical species classification only in 7 yeast strains. Using digestion of the ITS region, 26 strains, including allof lager yeast were identified as S. cerevisiae; this region is thus unsuitable for identification of industrial yeast strains. Identification of ale and lager yeast should partially reflect the maximum temperature of growth.
Czech name
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Czech description
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Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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