Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00069595" target="_blank" >RIV/00216224:14310/13:00069595 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s12223-013-0254-6" target="_blank" >http://dx.doi.org/10.1007/s12223-013-0254-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12223-013-0254-6" target="_blank" >10.1007/s12223-013-0254-6</a>

Result language
angličtina
Original language name
Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases
Original language description
Early childhood caries (ECC) has become a serious medical problem worldwide in the last decade. Bacterial microflora of the dental plaque and oral cavity is considered an important factor in the formation and progression of dental caries. The aim of thisstudy was strain typing and comparison of bacterial isolates retrieved from caries lesions and root canal contents of the same teeth. In total, 18 pairs of presumptive streptococci and lactobacilli retrieved from dental caries and root canals isolated from ECC-affected children, were selected on the basis of biotyping results and rep-PCR fingerprinting with (GTG)5 primer. Strain typing was further done using the RiboPrinter microbial characterization system (DuPont Qualicon). The automated ribotyping determined 14 pairs of the strains (77.8 %) to be identical. The results obtained confirmed that identical bacterial strains colonized both the decayed dental surface and the necrotic content of the dental pulp cavity during the cariogenes
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
<a href="/en/project/EE2.3.20.0183" target="_blank" >EE2.3.20.0183: Center of Experimental Biomedicine</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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