Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00073256" target="_blank" >RIV/00216224:14310/14:00073256 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity
Original language description
Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteriaof the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality wi
Czech name
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Czech description
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Project
<a href="/en/project/QJ1210047" target="_blank" >QJ1210047: Research and development of novel agents for honey bee health improvement</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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