Structural and Functional Analysis of a Novel Haloalkane Dehalogenase with Two Halide-Binding Sites.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00074206" target="_blank" >RIV/00216224:14310/14:00074206 - isvavai.cz</a>
Alternative codes found
RIV/67179843:_____/14:00430522 RIV/61388963:_____/14:00430522
Result on the web
<a href="http://dx.doi.org/10.1107/S1399004714009018" target="_blank" >http://dx.doi.org/10.1107/S1399004714009018</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1107/S1399004714009018" target="_blank" >10.1107/S1399004714009018</a>
Alternative languages
Result language
angličtina
Original language name
Structural and Functional Analysis of a Novel Haloalkane Dehalogenase with Two Halide-Binding Sites.
Original language description
Crystal structure of novel haloalkane dehalogenase DbeA revealed the presence of two chloride ions buried in the protein interior. The first halide-binding site is involved in substrate binding and is present in all structurally characterized haloalkanedehalogenases. The second halide-binding site is unique to DbeA. To elucidate the role of the second halide-binding site in enzyme functionality, a two-point mutant lacking this site was constructed and characterized. These substitutions resulted in a shift of substrate-specificity class and were accompanied by decrease of enzyme activity, stability and elimination of the substrate inhibition. The changes in enzyme catalytic activity were attributed to deceleration of the rate-limiting hydrolytic step,mediated by lower basicity of the catalytic histidine.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Crystallographica D
ISSN
0907-4449
e-ISSN
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Volume of the periodical
70
Issue of the periodical within the volume
July
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
1884-1897
UT code for WoS article
000338917000009
EID of the result in the Scopus database
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