Fructose 1-Phosphate is the One and Only Physiological Effector of the Cra (FruR) regulator of Pseudomonas putida.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00074207" target="_blank" >RIV/00216224:14310/14:00074207 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.fob.2014.03.013" target="_blank" >http://dx.doi.org/10.1016/j.fob.2014.03.013</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fob.2014.03.013" target="_blank" >10.1016/j.fob.2014.03.013</a>

Result language
angličtina
Original language name
Fructose 1-Phosphate is the One and Only Physiological Effector of the Cra (FruR) regulator of Pseudomonas putida.
Original language description
Fructose-1-phosphate (F1P) is the preferred effector of the catabolite repressor/activator (Cra) protein of the soil bacterium Pseudomonas putida but its ability to bind other metabolic intermediates in vivo is unclear. The Cra protein of this microorganism (CraPP) was submitted to mobility shift assays with target DNA sequences (the PfruB promoter) and candidate effectors fructose-1,6-biphosphate (FBP), glucose 6-phosphate (G6P), and fructose-6-phosphate (F6P). 1mM F1P was sufficient to release most ofthe Cra protein from its operators but more than 10mM of FBP or G6P was required to free the same complex. However, isothermal titration microcalorimetry failed to expose any specific interaction between CraPP and FBP or G6P. To solve this paradox, transcriptional activity of a PfruB-lacZ fusion was measured in wild-type and fruB cells growing on substrates that change the intracellular concentrations of F1P and FBP.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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