Microscopic Monitoring Provides Information on Structure and Properties During Biocatalyst Immobilisation.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00074208" target="_blank" >RIV/00216224:14310/14:00074208 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/14:00061048
Result on the web
<a href="http://dx.doi.org/10.1002/biot.201300049" target="_blank" >http://dx.doi.org/10.1002/biot.201300049</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/biot.201300049" target="_blank" >10.1002/biot.201300049</a>
Alternative languages
Result language
angličtina
Original language name
Microscopic Monitoring Provides Information on Structure and Properties During Biocatalyst Immobilisation.
Original language description
Enzymes have a wide range of applications in different industries owing to their high specificity and efficiency. Immobilisation is often used to improve biocatalyst properties, operational stability and reusability. However, changes in the structure ofbiocatalysts during immobilisation and under process conditions are still largely uncertain. Here, three microscopy techniques ? bright-field, confocal and electron microscopy ? were applied to determine the distribution and structure of an immobilised biocatalyst. Free enzyme (haloalkane dehalogenase), cross-linked enzyme aggregates (CLEAs) and CLEAs entrapped in polyvinyl alcohol lenses (lentikats) were used as model systems. Electron microscopy revealed that sonicated CLEAs underwent morphological changes that strongly correlated with increased catalytic activity compared to less structured, non-treated CLEAs. Confocal microscopy confirmed that loading of the biocatalyst was not the only factor affecting the catalytic activity of the
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biotechnology Journal
ISSN
1860-6768
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
6
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
852-860
UT code for WoS article
000337700000015
EID of the result in the Scopus database
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