SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00083392" target="_blank" >RIV/00216224:14310/14:00083392 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION
Original language description
Laser induced fluorescence capillary electrophoresis (CE-LIF) with 515 nm green laser module was used for sensitive determination of glutathione (GSH) in various biological samples. For the first time, eosin-5-maleimide was used as a fluorescent tag in CE. Studies were performed to optimize the derivatization conditions (the ratio of reagent to analyte, the reaction time, temperature, etc.). CE-LIF also offers a unique opportunity to monitor the stability of the reagent and various reaction products. Wedemonstrate the applicability of the developed method on determination of GSH in real biological samples (exhaled breath condensate and saliva) with LODs as low as 180 pM.
Czech name
—
Czech description
—

Project
—
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS
ISBN
9788090495920
ISSN
—
e-ISSN
—
Number of pages
5
Pages from-to
232-236
Publisher name
INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC
Place of publication
BRNO
Event location
Brno, CZECH REPUBLIC
Event date
Oct 20, 2014
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000354547400077

Similar results(10)