NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC FIBROSIS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00083393" target="_blank" >RIV/00216224:14310/14:00083393 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC FIBROSIS
Original language description
Capillary electrophoresis (CE) with double opposite end injection (DOEI) and contactless conductivity detection (C4D) was used in a novel approach for diagnosis of cystic fibrosis (CF). A simple, fast and inexpensive "skin wipe" technique for sweat sampling was developed as a time-saving substitute for conventional, iontophoretic sweat sampling. Saliva sample was also obtained. A number of major target analytes (inorganic/organic anions and inorganic cations) were quantified simultaneously in these samples. By applying principal component analysis (PCA) to ion concentration ratios, rather than to individual ion concentrations, more accurate diagnostic tool for CF was obtained. We demonstrate that by using the developed approach and comparing a group ofhealthy individuals to patients diagnosed with CF, two clearly distinguished clusters can be observed without any data overlap. This approach may thus set a basis for a new method to diagnose CF more reliably.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS
ISBN
9788090495920
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
182-186
Publisher name
INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC
Place of publication
BRNO
Event location
Brno, CZECH REPUBLIC
Event date
Oct 20, 2014
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000354547400061