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Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00080624" target="_blank" >RIV/00216224:14310/15:00080624 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1002/elps.201400487" target="_blank" >http://dx.doi.org/10.1002/elps.201400487</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/elps.201400487" target="_blank" >10.1002/elps.201400487</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection

  • Original language description

    Importance of human assisted reproduction is rising as a response for rising number of couples suffering from infertility. The key steps in human assisted reproduction are in vitro fertilization of an oocyte, cultivation of embryo and selection of an embryo with the highest developmental potential for implantation. Assessment of developmental potential of embryo is lacking of precision thus novel techniques are sought. Capillary electrophoresis with contactless conductivity detection was applied for analysis of pyruvate and lactate as potential biomarkers of developmental potential of human embryos. Optimizations of separation conditions and treatment of a sample are described and discussed. Developed method was successfully applied for analysis of several samples of spent culture media collected after cultivation of embryos.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Electrophoresis

  • ISSN

    0173-0835

  • e-ISSN

  • Volume of the periodical

    36

  • Issue of the periodical within the volume

    11-12

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    7

  • Pages from-to

    1244-1250

  • UT code for WoS article

    000356004200003

  • EID of the result in the Scopus database