Novel LinA Type 3 delta-Hexachlorocyclohexane Dehydrochlorinase
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00085588" target="_blank" >RIV/00216224:14310/15:00085588 - isvavai.cz</a>
Result on the web
<a href="http://aem.asm.org/content/81/21/7553" target="_blank" >http://aem.asm.org/content/81/21/7553</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/AEM.01683-15" target="_blank" >10.1128/AEM.01683-15</a>
Alternative languages
Result language
angličtina
Original language name
Novel LinA Type 3 delta-Hexachlorocyclohexane Dehydrochlorinase
Original language description
LinA is the first enzyme of the microbial degradation pathway of a chlorinated insecticide, hexachlorocyclohexane (HCH), and mediates the dehydrochlorination of alpha-, gamma-, and delta-HCH. Its two variants, LinA type 1 and LinA type 2, which differ at10 out of 156 amino acid residues, have been described. Their activities for the metabolism of different HCH isomers differ considerably but overall are high for gamma-HCH, moderate for alpha-HCH, low for delta-HCH, and lacking for beta-HCH. Here, we describe the characterization of a new variant of this enzyme, LinA type 3, whose gene was identified from the metagenome of an HCH-contaminated soil sample. Its deduced primary structure in the region spanning amino acid residues 1 to 147 of the protein exhibits 17 and 12 differences from LinA type 1 and LinA type 2, respectively. In addition, the residues GIHFAPS, present at the region spanning residues 148 to 154 in both LinA type 1 and LinA type 2, are deleted in LinA type 3.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied and Environmental Microbiology
ISSN
0099-2240
e-ISSN
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Volume of the periodical
81
Issue of the periodical within the volume
21
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
7553-7559
UT code for WoS article
000363462900021
EID of the result in the Scopus database
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