Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00085836" target="_blank" >RIV/00216224:14310/15:00085836 - isvavai.cz</a>
Alternative codes found
RIV/67985904:_____/15:00457471
Result on the web
<a href="http://dx.doi.org/10.5604/12321966.1185766" target="_blank" >http://dx.doi.org/10.5604/12321966.1185766</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5604/12321966.1185766" target="_blank" >10.5604/12321966.1185766</a>
Alternative languages
Result language
angličtina
Original language name
Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic
Original language description
During 2008-2012, a total of 466 ticks Ixodes ricinus removed from humans were collected and tested for the presence of Borrelia burgdorferi sensu lato (Bbsl). Ticks were collected in all districts of the South Moravian region of the Czech Republic (CZ).The aim of this study was to determine the infestation of Bbsl in ticks Ixodes ricinus and the identification of genospecies of Bbsl group by DNA sequencing. DNA isolation from homogenates was performed by UltraClean BloodSpin DNA kit (MoBio) and by automated instrument Prepito (Perkin-Elmer). Detection of spirochetes was carried out by RealTime PCR kit EliGene Borrelia LC (Elisabeth Pharmacon). Finally, all the positive samples were sequenced on an ABI 3130 Genetic Analyzer (Life Technologies) and identified in the BLAST (NCBI) database. A total positivity of the samples was 26%. For the first time in the Czech Republic, 5 of the isolated strains were genotyped as Borrelia spielmanii (7.1%). Other representatives of Bbsl were also obs
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Annals of Agricultural and Environmental Medicine
ISSN
1232-1966
e-ISSN
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Volume of the periodical
22
Issue of the periodical within the volume
4
Country of publishing house
PL - POLAND
Number of pages
5
Pages from-to
637-641
UT code for WoS article
000369231000011
EID of the result in the Scopus database
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