Polymer-immobilized ready-to-use recombinant yeast assays for the detection of endocrine disruptive compounds
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00086633" target="_blank" >RIV/00216224:14310/15:00086633 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0045653515001873" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0045653515001873</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chemosphere.2015.02.063" target="_blank" >10.1016/j.chemosphere.2015.02.063</a>
Alternative languages
Result language
angličtina
Original language name
Polymer-immobilized ready-to-use recombinant yeast assays for the detection of endocrine disruptive compounds
Original language description
Recombinant yeast assays (RYAs) constitute a suitable tool for the environmental monitoring of compounds with endocrine disrupting activities, notably estrogenicity and androgenicity. Conventional procedures require yeast reconstitution from frozen stock, which usually takes several days and demands additional equipment With the aim of applying such assays to field studies and making them more accessible to less well-equipped laboratories, we have optimized RYA by the immobilization of Saccharomyces cerevisiae cells in three different polymer matrices gelatin, Bacto agar, and Yeast Extract Peptone Dextrose agar to obtain a ready-to-use version for the fast assessment of estrogenic and androgenic potencies of compounds and environmental samples. Among the three matrices, gelatin showed the best results for both testosterone (androgen receptor yeast strain; AR-RYA) and 17 beta-estradiol (estrogen receptor yeast strain; ER-RYA).
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
DN - Environmental impact on health
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LO1214" target="_blank" >LO1214: Research Centre for Toxic Compounds in the Environment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemosphere
ISSN
0045-6535
e-ISSN
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Volume of the periodical
132
Issue of the periodical within the volume
AUGUST
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
56-62
UT code for WoS article
000355882900008
EID of the result in the Scopus database
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