Identification and Characterization of Androgen-Responsive Genes in Zebrafish Embryos

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00086676" target="_blank" >RIV/00216224:14310/15:00086676 - isvavai.cz</a>
Result on the web
<a href="http://pubs.acs.org/doi/10.1021/acs.est.5b01034" target="_blank" >http://pubs.acs.org/doi/10.1021/acs.est.5b01034</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.est.5b01034" target="_blank" >10.1021/acs.est.5b01034</a>

Result language
angličtina
Original language name
Identification and Characterization of Androgen-Responsive Genes in Zebrafish Embryos
Original language description
Responsive genes for fish embryos have been identified so far for some endocrine pathways but not for androgens. Using transcriptome analysis and multiple concentration response modeling, we identified putative androgen-responsive genes in zebrafish embryos exposed to 0.05-5000 nM 11-ketotestosterone for 24 h. Four selected genes with sigmoidal concentration-dependent expression profiles (EC50 = 6.5-30.0 nM) were characterized in detail. The expression of cyp2k22 and slco1f4 was demonstrated in the pronephros; lipca was detected in the liver, and sult2st3 was found in the olfactory organs and choroid plexus. Their expression domains, the function of human orthologs, and a pathway analysis suggested a role of these genes in the metabolism of hormones. Hence, it was hypothesized that they were induced to compensate for elevated hormone levels.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
DN - Environmental impact on health
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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