PHYLOGENETIC ANALYSIS OF CULTIVATION-RESISTANT TERRESTRIAL CYANOBACTERIA WITH MASSIVE SHEATHS (STIGONEMA SPP. AND PETALONEMA ALATUM, NOSTOCALES, CYANOBACTERIA) USING SINGLE-CELL AND FILAMENT SEQUENCING OF ENVIRONMENTAL SAMPLES
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00087123" target="_blank" >RIV/00216224:14310/15:00087123 - isvavai.cz</a>
Alternative codes found
RIV/67985939:_____/15:00447943 RIV/60076658:12310/15:43888731
Result on the web
<a href="http://dx.doi.org/10.1111/jpy.12273" target="_blank" >http://dx.doi.org/10.1111/jpy.12273</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jpy.12273" target="_blank" >10.1111/jpy.12273</a>
Alternative languages
Result language
angličtina
Original language name
PHYLOGENETIC ANALYSIS OF CULTIVATION-RESISTANT TERRESTRIAL CYANOBACTERIA WITH MASSIVE SHEATHS (STIGONEMA SPP. AND PETALONEMA ALATUM, NOSTOCALES, CYANOBACTERIA) USING SINGLE-CELL AND FILAMENT SEQUENCING OF ENVIRONMENTAL SAMPLES
Original language description
Molecular assessment of a large portion of traditional cyanobacterial taxa has been hindered by the failure to isolate and grow them in culture. In this study, we developed an optimized protocol for single cell/filament isolation and 16S rRNA gene sequencing of terrestrial cyanobacteria with large mucilaginous sheaths, and applied it to determine the phylogenetic position of typical members of the genera Petalonema and Stigonema. A methodology based on a glass-capillary isolation technique and a semi-nested PCR protocol enabled reliable sequencing of the 16S rRNA gene from all samples analysed. Ten samples covering 7 species of Stigonema from Europe, North and Central America, and Hawaii, and the type species of Petalonema from Slovakia were sequenced.Contrary to some previous studies, which proposed a relation to heteropolar nostocalean cyanobacteria, Petalonema appeared to belong to the family Scytonemataceae.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Phycology
ISSN
0022-3646
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
288-297
UT code for WoS article
000352617500009
EID of the result in the Scopus database
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