Root and Eruption Defects in c-Fos Mice Are Driven by Loss of Osteoclasts

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00113937" target="_blank" >RIV/00216224:14310/15:00113937 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/15:00457861 RIV/62157124:16170/15:43873744

Result on the web

<a href="https://doi.org/10.1177/0022034515608828" target="_blank" >https://doi.org/10.1177/0022034515608828</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1177/0022034515608828" target="_blank" >10.1177/0022034515608828</a>

Result language

angličtina

Original language name

Root and Eruption Defects in c-Fos Mice Are Driven by Loss of Osteoclasts

Original language description

c-Fos homozygous mice lack osteoclasts with a failure of the teeth to erupt and with an arrest of root development. Here, we characterize the defects associated with the failure in root development and the loss of the tooth-bone interface, and we investigate the underlying causes. We show that, while homozygous c-Fos mice have no multinucleated osteoclasts, heterozygous mice have a reduction in the number of osteoclasts with a reduction in the tooth-bone interface during development and subtle skeletal defects postnatally. In the homozygous mutants bone is found to penetrate the tooth, particularly at the apical end, physically disrupting the root forming HERS (Hertwig's epithelial root sheath) cells. The cells of the HERS continue to proliferate but cannot extend downward due to the presence of bone, leading to a loss of root formation. Tooth germ culture showed that the developing tooth invaded the static bone in mutant tissue, rather than the bone encroaching on the tooth. Although c-Fos has been shown to be expressed in developing teeth, the defect in maintenance of the tooth-bone interface appears to be driven solely by the lack of osteoclasts, as this defect can be rescued in the presence of donor osteoclasts. The rescue suggests that signals from the tooth recruit osteoclasts to clear the bone from around the tooth, allowing the tooth to grow, form roots, and later erupt.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30208 - Dentistry, oral surgery and medicine

Project

<a href="/en/project/GB14-37368G" target="_blank" >GB14-37368G: Centre of orofacial development and regeneration</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Dental Research

ISSN

0022-0345

e-ISSN

1544-0591

Volume of the periodical

94

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

1724-1731

UT code for WoS article

000365334400014

EID of the result in the Scopus database

2-s2.0-84948416012