Impact of Interspecific Hybridization of T. pratense x T. medium and Backcrossing on Genetic Variability of Progeny

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087683" target="_blank" >RIV/00216224:14310/16:00087683 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.17221/115/2016-CJGPB" target="_blank" >http://dx.doi.org/10.17221/115/2016-CJGPB</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/115/2016-CJGPB" target="_blank" >10.17221/115/2016-CJGPB</a>

Result language
angličtina
Original language name
Impact of Interspecific Hybridization of T. pratense x T. medium and Backcrossing on Genetic Variability of Progeny
Original language description
Red clover (Trifolium pratense L.) is a high-quality fodder crop which has been hybridized successfully with its wild relative zigzag clover (T. medium L.). The aim of this study was to evaluate the genetic impact of interspecific hybridization and subsequent repeated backcrossing on the variability within hybrid progeny genomes. Nuclear DNA content of 800 and 753 hybrid plants from F-7/F-8 and F-8/F-9 generations, respectively, was measured by flow cytometry. Resulting values were converted to estimated chromosome counts, which were successfully validated on a sample of 28 plants by counting mitotic chromosomes. The two generations showed a similar distribution of various chromosome counts ranging from 22 to 47 chromosomes. In total, 24.0% and 34.3% of plants from the two generations had different numbers of chromosomes from their parental plants. Variability within the hybrid population was assessed by fluorescent in situ hybridization using rDNA probes.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/QI111A019" target="_blank" >QI111A019: New genomic technology for the alogamic cultivated plant breeding for improving utility traits.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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