Exploring the transcriptome of Eudiplozoon nipponicum (Monogenea, Diplozoidae) adult worm
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087937" target="_blank" >RIV/00216224:14310/16:00087937 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Exploring the transcriptome of Eudiplozoon nipponicum (Monogenea, Diplozoidae) adult worm
Original language description
Parasitic monogeneans are blood sucking fish ectoparasites. Their presence can lead to significant losses in fish stocks. From a large group of Monogenea (ca 25 000 species) only two species have been deeply sequenced – genomes of Gyrodactylus salaris and Protopolystoma xenopodis and several monogenean protein molecules have been characterized (e.g. cathepsin L – E. nipponicum and Neobenedenia melleni and three unknown serine peptidases from Neobenedenia girellae). In our study of E. nipponicum transcriptome we adopted two different sequencing approaches. 454 pyrosequencing method (GS FLX System, Roche) and sequencing by synthesis method (GS MiSeq, Illumina) to obtain high quality transcriptomic data. We generated 324 941 reads which were assembled into 6910 contigs (454 GS FLX System) and 149 697 864 reads assembled into almost 10 000 unique sequences (Illumina MiSeq) after processing by specific software tools.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EG - Zoology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP505%2F12%2FG112" target="_blank" >GBP505/12/G112: ECIP - European Centre of Ichtyoparasitology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů