Structure and function of Photorhabdus asymbiotica lectins: Studies of potential virulence factors from emerging human pathogen

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088181" target="_blank" >RIV/00216224:14310/16:00088181 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Structure and function of Photorhabdus asymbiotica lectins: Studies of potential virulence factors from emerging human pathogen
Original language description
Lectins are ubiquitous carbohydrate-binding proteins found in bacteria, plants, animals, fungi and viruses where they play a key role in various processes. Our research is focused on lectins from Photorhabdus genus - gram-negative bioluminescent bacteria. Photorhabdus genus contains three species all of which are insect pathogens: P. luminescens, P. temperata and P. asymbiotica. Unlike other species in this genus, P. asymbiotica is also able to infect humans. In the P. asymbiotica genome, three genes of putative lectins named PHL, PHL2 and PHL3 were found. Recombinant lectins were studied using broad range of biophysical methods. For affinity measurements we employed isothermal titration calorimetry, micro-scale thermophoresis and surface plasmon resonance. The oligomeric state of the proteins was determined by analytical ultracentrifugation. We have optimized crystallization conditions using high throughput screening, and diffracting-quality crystals were used for structure determination.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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