BACE enzyme kinetics detected by MALDI-TOF mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088259" target="_blank" >RIV/00216224:14310/16:00088259 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
BACE enzyme kinetics detected by MALDI-TOF mass spectrometry
Original language description
Matrix-assisted laser desorption/ionization mass spectrometry was introduced for the analysis of enzyme kinetics and inhibition. The Km for selected substrate and IC50 values for enzyme inhibitor were in a good agreement with results obtained by capillary electrophoresis coupled to electrospray mass spectrometry. More specifically, it was focused on beta secretase (BACE), an enzyme playing important role in Alzheimer’s disease development. Its selective inhibition in brain could be beneficial for the patients; thus, specific inhibitor of this enzyme was examined as well. In comparison with traditional fluorometric or colorimetric methods, the both mass spectrometric approaches offered faster, sensitive and more precise BACE analysis.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
CECE 2016
ISBN
9788090495944
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
186-188
Publisher name
Institute of Analytical Chemistry of the CAS, v. v. i., Brno, Czech Republic
Place of publication
Brno
Event location
Brno
Event date
Jan 1, 2016
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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