De-novo development of microsatellite markers for Dactylogyrus vistulae

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088713" target="_blank" >RIV/00216224:14310/16:00088713 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
De-novo development of microsatellite markers for Dactylogyrus vistulae
Original language description
In our study, we aimed to characterize de-novo microsatellite markers for Dactylogyrus parasites. Dactylogyrus vistulae was selected as a model organism owing to its relatively large body size in comparison to the other members of this genus. Approximately 300 specimens of D. vistulae were collected from European chub Squalius cephalus of the Dyje River. Genomic DNA was isolated and subsequently prepared genomic libraries were subjected to Illumina MiSeq (provided by CEITEC, MU, Brno). QDD program (Meglécz et al. 2010) was used for selecting microsatellite markers and primer design. Following criteria used in Gettová et al. (2013) 70 sequences with target microsatellites were selected. Monogenean parasites, especially dactylogyrids, display a high level of host specificity. As a result genetic structure of monogenean parasites might be host-dependent. Microsatellite markers developed for Dactylogyrus parasites might, therefore, enable us to reconstruct the evolution history of their fish hosts.
Czech name
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Czech description
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Project
<a href="/en/project/GAP505%2F12%2F0375" target="_blank" >GAP505/12/0375: Evolutionary-immunological and ecological aspects of parasitism in hybrid and polyploid cyprinid fish</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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