Molecular and biochemical characterization of cysteine peptidase inhibitor from Eudiplozoon nipponicum (Monogenea)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00093421" target="_blank" >RIV/00216224:14310/16:00093421 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Molecular and biochemical characterization of cysteine peptidase inhibitor from Eudiplozoon nipponicum (Monogenea)
Original language description
Inhibitors of cysteine peptidases (cystatins) are proteins produced by a wide range of organisms, including parasites. Besides regulation of the basic physiological functions of parasites they may act either as modulators of the host immune system or regulators of blood-digestion. We identified that cystatin genes are expressed and their protein form probably secreted by Eudiplozoon nipponicum. In following experiments we focused on molecular and functional characterization of cystatin from E. nipponicum (Monogenea). We performed bacterial expression of E. nipponicum cystatin gene (pET19b plasmid vector, E. coli BL21DE3 RIPL cells) and purified the recombinant protein [1]. Phylogenetic trees inferred using Bayesian inference, maximum likelihood, neighbour-joining methods revealed close phylogenetic relationship with cystatins of cestodes.
Czech name
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Czech description
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Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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