Transient suppression of gap junctional intercellular communication after exposure to 100-nanosecond pulsed electric fields

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00093524" target="_blank" >RIV/00216224:14310/16:00093524 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S1567539416300871" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1567539416300871</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioelechem.2016.07.003" target="_blank" >10.1016/j.bioelechem.2016.07.003</a>

Result language

angličtina

Original language name

Transient suppression of gap junctional intercellular communication after exposure to 100-nanosecond pulsed electric fields

Original language description

Gap junctional intercellular communication (GJIC) is an important mechanism that is involved and affected in many diseases and injuries. So far, the effect of nanosecond pulsed electric fields (nsPEFs) on the communication between cells was not investigated. An in vitro approach is presented with rat liver epithelial WB-F344 cells grown and exposed in a monolayer. In order to observe sub-lethal effects, cells were exposed to pulsed electric fields with a duration of 100 ns and amplitudes between 10 and 20 kV/cm. GJIC strongly decreased within 15 min after treatment but recovered within 24 h. Gene expression of Cx43 was significantly decreased and associated with a reduced total amount of Cx43 protein. In addition, MAP kinases p38 and Erk1/2, involved in Cx43 phosphorylation, were activated and Cx43 became hyperphosphorylated. Immunofluorescent staining of Cx43 displayed the disassembly of gap junctions.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioelectrochemistry

ISSN

1567-5394

e-ISSN

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Volume of the periodical

112

Issue of the periodical within the volume

December

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

33-46

UT code for WoS article

000383523800005

EID of the result in the Scopus database

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