Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00094239" target="_blank" >RIV/00216224:14310/16:00094239 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3791/54281" target="_blank" >http://dx.doi.org/10.3791/54281</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3791/54281" target="_blank" >10.3791/54281</a>
Alternative languages
Result language
angličtina
Original language name
Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
Original language description
This protocol describes a scalpel loading-fluorescent dye transfer (SL-DT) technique that measures intercellular communication through gap junction channels, which is a major intercellular process by which tissue homeostasis is maintained. Interruption of gap junctional intercellular communication (GJIC) by toxicants, toxins, drugs, etc. has been linked to numerous adverse health effects. Many genetic-based human diseases have been linked to mutations in gap junction genes. The SL-DT technique is a simple functional assay for the simultaneous assessment of GJIC in a large population of cells. The assay involves pre-loading cells with a fluorescent dye by briefly perturbing the cell membrane with a scalpel blade through a population of cells. The fluorescent dye is then allowed to traverse through gap junction channels to neighboring cells for a designated time. The assay is then terminated by the addition of formalin to the cells.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
DN - Environmental impact on health
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
ISSN
1940-087X
e-ISSN
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Volume of the periodical
2016
Issue of the periodical within the volume
118
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
"e54281"-"e54289"
UT code for WoS article
000397846600008
EID of the result in the Scopus database
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