Monogenea: Parasites of fish and their unknown molecules

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00094614" target="_blank" >RIV/00216224:14310/17:00094614 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Monogenea: Parasites of fish and their unknown molecules

Original language description

The majority of known monogenean species are ectoparasites of fish with monoxenous life cycle in freshwater or marine ecosystems. Some species are of high economic importance, they can be highly pathogenic to fish, which can result in losses in aquaculture. In contrast to these facts there is almost nothing known about the molecular nature of the interactions with fish hosts and functions of particular monogeneans bioactive molecules. In a first part of our pilot study of monogeneans molecules we adopted the NGS techniques in order to get the high quality “monogenean-polyopisthocotylean genome/transcriptome matrix”. In a second part of our study this platform was used for the identification of the monogeneans´ dominant protein molecules followed by their further molecular/biochemical characterization. We adopted experimental organism Eudiplozoon nipponicum (Platyhelminthes, Monogenea) in order to obtain high quality genome, transcriptome and proteome for further annotation, development of robust gene/protein muster platform for this group of organisms. Subsequently, we used this dataset in combination with bioinformatics approach enable in silico determination of antigenic proteins - vaccine candidates. In addition to the bioinformatics analyses we performed mass spectrometric identification of particular protein compounds in excretory-secretory products from E. nipponicum in order to confirm the presence of previously predicted antigenic molecules. Finally, some of the predicted molecules have been prepared in recombinant form and characterized (serpins, cystatins).

Czech name

—

Czech description

—

Type

O - Miscellaneous

CEP classification

—

OECD FORD branch

10600 - Biological sciences

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů