Concurrent infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis in two types of porcine macrophages: apoptosis, production of ROS and formation of multinucleated giant cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00108732" target="_blank" >RIV/00216224:14310/17:00108732 - isvavai.cz</a>

Alternative codes found

RIV/00027162:_____/17:N0000024

Result on the web

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418695/pdf/13567_2017_Article_433.pdf" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418695/pdf/13567_2017_Article_433.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13567-017-0433-6" target="_blank" >10.1186/s13567-017-0433-6</a>

Result language

angličtina

Original language name

Concurrent infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis in two types of porcine macrophages: apoptosis, production of ROS and formation of multinucleated giant cells

Original language description

Porcine reproductive and respiratory syndrome (PRRS) is one of the most signifcant and economically important infectious diseases afecting swine worldwide and can predispose pigs to secondary bacterial infections caused by, e.g. Haemophilus parasuis. The aim of the presented study was to compare susceptibility of two diferent types of macrophages which could be in contact with both pathogens during infection with PRRS virus (PRRSV) and in co-infection with H. parasuis. Alveolar macrophages (PAMs) as resident cells provide one of the frst lines of defence against microbes invading lung tissue. On the other hand, monocyte derived macrophages (MDMs) represent infammatory cells accumulating at the site of infammation. While PAMs were relatively resistant to cytopathogenic efect caused by PRRSV, MDMs were much more sensitive to PRRSV infection. MDMs infected with PRRSV increased expression of pro-apoptotic Bad, Bax and p53 mRNA. Increased mortality of MDMs may be also related to a higher intensity of ROS production after infection with PRRSV. In addition, MDMs (but not PAMs) infected with H. parasuis alone formed multinucleated giant cells (MGC); these cells were not observed in MDMs infected with both pathogens. Higher sensitivity of MDMs to PRRSV infection, which is associated with limited MDMs survival and restriction of MGC formation, could contribute to the development of multifactorial respiratory disease of swine.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30102 - Immunology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Research

ISSN

0928-4249

e-ISSN

1297-9716

Volume of the periodical

48

Issue of the periodical within the volume

MAY

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

28

UT code for WoS article

000401466700001

EID of the result in the Scopus database

2-s2.0-85018769465