The forebody organisation in Paradiplozoon homoion: A microscopic study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00100949" target="_blank" >RIV/00216224:14310/18:00100949 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The forebody organisation in Paradiplozoon homoion: A microscopic study
Original language description
The family Diplozoidae (Monogenea), including investigated species Paradiplozoon homoion, represent blood-feeding gill ectoparasites of freshwater fish. They exhibit unusual body organisation and life cycle involving a permanent fusion of two larval worms and their subsequent transformation into one individual. Using a combined approach of confocal and electron microscopy, this study focused on the parasite forebody. Special attention was given to parasite’s nervous, excretory and digestive systems. Labelling of filamentous actin with fluorescent phalloidin revealed the musculature of the body wall, buccal suckers and the pharynx. Staining with chloride carmine or counterstaining with DAPI proved to be powerful tools for confocal microscopic detection of various cell types including putative gland cells. Immunofluorescent detection of alfa-tubulin showed to be suitable for visualisation of excretory system and some parts of nervous system.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
<a href="/en/project/GBP505%2F12%2FG112" target="_blank" >GBP505/12/G112: ECIP - European Centre of Ichtyoparasitology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů