Determination of in vitro isoflavone degradation in rumen fluid

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00101026" target="_blank" >RIV/00216224:14310/18:00101026 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3168/jds.2017-13610" target="_blank" >http://dx.doi.org/10.3168/jds.2017-13610</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3168/jds.2017-13610" target="_blank" >10.3168/jds.2017-13610</a>

Result language
angličtina
Original language name
Determination of in vitro isoflavone degradation in rumen fluid
Original language description
The aim of this study was to determine the degradation of dietary isoflavones in rumen fluid under 2 feeding regimens. The experiments were performed in vitro using a rumen fluid buffer system. The rumen fluid was taken from cows fed either a hay diet or a concentrate-rich diet (the diet consisted of 34.6% maize silage, 17.6% haylage, 12.8% alfalfa hay, and 35.0% supplemental mixture on a dry matter basis). As a source of isoflavones, 40% soybean extract (Biomedica, Prague, Czech Republic) at levels of 5, 25, 50, and 75 mg per 40 mL of rumen fluid was used. Samples of soybean extract were incubated in triplicate at 39 degrees C for 0, 3.0, 6.0, 12.0, arid 24.0 h in incubation solution. The metabolism of daidzein and genistein was faster under concentrate-rich diet conditions. In general, production of equol started after 3 to 6 h of incubation and reached the highest rate after approximately 12 h of incubation regardless of the type of diet or concentration of extract. In most of the experiments, production of equol continued after 24 h of incubation. Generally, equol production was greater under the hay diet conditions. Furthermore, experiments with higher amounts of added soybean extract revealed possible inhibitory effects of high levels of isoflavones on the rumen microflora.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project
<a href="/en/project/GA13-34134S" target="_blank" >GA13-34134S: Metabolism of Isoflavones in Rumen with respect to Equol Content in Bovine Milk</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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