Structure and properties of AB21, a novel Agaricus bisporus protein with structural relation to bacterial pore-forming toxins
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00106526" target="_blank" >RIV/00216224:14310/18:00106526 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/prot.25522" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/prot.25522</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/prot.25522" target="_blank" >10.1002/prot.25522</a>
Alternative languages
Result language
angličtina
Original language name
Structure and properties of AB21, a novel Agaricus bisporus protein with structural relation to bacterial pore-forming toxins
Original language description
We report the characterization of the dimeric protein AB21 from Agaricus bisporus, one of the most commonly and widely consumed mushrooms in the world. The protein shares no significant sequence similarity with any protein of known function, and it is the first characterized member of its protein family. The coding sequence of the ab21 gene was determined and the protein was expressed in E. coli in a recombinant form. We demonstrated a high thermal and pH stability of AB21 and proved the weak affinity of the protein to divalent ions of some transition metals (nickel, zinc, cadmium, and cobalt). The reported crystallographic structure exhibits an interesting rodlike helical bundle fold with structural similarity to bacterial toxins of the ClyA superfamily. By immunostaining, we demonstrated an abundance of AB21 in the fruiting bodies of A. bisporus.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proteins : structure, function, and genetics
ISSN
0887-3585
e-ISSN
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Volume of the periodical
86
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
897-911
UT code for WoS article
000446996700001
EID of the result in the Scopus database
2-s2.0-85054647931