Single-molecule localization microscopy as a promising tool for gamma H2AX/53BP1 foci exploration
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00107875" target="_blank" >RIV/00216224:14310/18:00107875 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/18:00495086
Result on the web
<a href="https://link.springer.com/article/10.1140%2Fepjd%2Fe2018-90148-1" target="_blank" >https://link.springer.com/article/10.1140%2Fepjd%2Fe2018-90148-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1140/epjd/e2018-90148-1" target="_blank" >10.1140/epjd/e2018-90148-1</a>
Alternative languages
Result language
angličtina
Original language name
Single-molecule localization microscopy as a promising tool for gamma H2AX/53BP1 foci exploration
Original language description
Quantification and structural studies of DNA double strand breaks (DSBs) are an essential part of radiobiology because DSBs represent the most serious damage introduced to the DNA molecule by ionizing radiation. Although standard immunofluorescence confocal microscopy has demonstrated its usefulness in a large number of research studies, it lacks the resolution required to separate individual, closely associated DSBs, which appear after cell exposure to high linear energy transfer (high-LET) radiation and can be visualized as clusters or streaks of radiation-induced repair foci (IRIFs). This prevents our deeper understanding of DSB induction and repair. Recent breakthroughs in super-resolution light microscopy, such as the development of single-molecule localization microscopy (SMLM), offer an optical resolution of approximately an order of magnitude better than that of standard confocal microscopy and open new horizons in radiobiological research. Unlike electron microscopy, SMLM (also referred to as "nanoscopy") preserves the natural structure of biological samples and is not limited to very thin sample slices. Importantly, SMLM not only offers a resolution on the order of approximately 10 nm, but it also provides entirely new information on the biochemistry and spatio-temporal organization of DSBs and DSB repair at the molecular level. Nevertheless, it is still challenging to correctly interpret these often surprising nanoscopy results. In the present article, we describe our first attempts to use SMLM to explore gamma H2AX and 53BP1 repair foci induced with( 15) N high-LET particles.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
The European Physical Journal D
ISSN
1434-6060
e-ISSN
1434-6079
Volume of the periodical
72
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
1-11
UT code for WoS article
000444642900001
EID of the result in the Scopus database
2-s2.0-85053394793